4 μl of ATP and 4 μl of enzymatic buffer with 5 μl of ADP labeled with the fluorophore d2 and 5 μl of Eu 3+ cryptate–labeled antibody to ADP. The first step of the assay development was an ...

and an increase in the ATP/ADP ratio (1); the increased ATP/ADP ratio leads to closing of the potassium channel and depolarization of the cell (2); and cell depolarization opens a calcium channel ...