The major difference from procedures described by others previously is the use of Matrigel in combination with feeder cell-conditioned medium (CM) to culture the cells in a true monolayer rather ...

Cell culture media are complex products that ... A good deal of hESC work was carried out in murine-conditioned media that contained components of mouse embryonic fibroblasts (MEF).

The thawed conditioned media was used to culture mouse ES cells in six-well plates that were incubated further (37ºC, 96 hours). As a control, six-well plates of mouse ES cells were incubated ...

We present a fully automated setup for performing in-line mass spectrometry (MS) analysis of conditioned media in cell cultures, in particular focusing on the peptides therein. The goal is to assess ...

By growing PEL cells in culture and analyzing the secretome (proteins secreted into cell-conditioned media), investigators have identified proteins that may explain PEL pathogenesis, its peculiar ...