The luminosity of the fluorescent signal from DAPI can increase almost 20-fold ... of the stain is in the violet region of the spectrum (i.e. 405 nm). Although it is conventionally used to stain ...

genetically modifies the protein of interest to make it fluorescent. Stains can also be added to a sample which binds specifically to the protein of interest. For example, DAPI is a nucleic acid ...

By nature, each fluorescent dye exhibits a unique absorbance and emission spectra, resulting from the change in energy states of electrons when the fluorophore is excited. These absorbance and ...

This is a fluorescent microscopy picture of cells of a human macrophage cell line (THP-1), which was incubated with particles (beads) stained with a blue and a red dye; the red dye is only active ...

The nuclei are counter stained and visualized using DAPI fluorescent dye. Multiplex assay is suitable for FFPE or fixed/fresh frozen sections as well as for fixed adherent cells. Dual RNA in situ ...

Fluorescence spectra are often subject to significant distortions. These are largely predictable and are caused by concentration-dependent Inner Filter Effects (IFE). It is therefore the ...

Ratiometric fluorescence is a method where the intensities at two or more wavelengths of an emission spectrum or excitation are measured in order to detect changes to a local environment. This is ...