An oligomerization-dependent system comprising two fragments of green fluorescent protein (GFP) fused to engineered zinc fingers could be a means to detect virtually any double-stranded DNA sequence.

The model was able to predict optimized GFP sequences that had as many as seven ... a viral vector that is commonly used to deliver DNA. In that case, they optimized the capsid for its ability ...

1B). In all cases, the flap sequence significantly enhanced GFP transduction mediated by the HIV vector. Figure 1: The HIV-1 central DNA flap enhances gene transduction of primary neural cells.

The precise insertion of DNA sequences using the homology-directed ... 21%), the KI efficiency was found to be 80% higher. GFP KI at RAB11A Locus in HEK293 cells using electroporation with 5 ...