The assay is run in two main steps, the kinase reaction step followed by the detection step with HTRF reagents (Fig. 1). The phosphorylation of the biotin-tagged generic peptide is initiated by ...
If the latter reactions were allowed to reach equilibrium ... the AMP-activated protein kinase (AMPK), with its relatives in other eukaryotic kingdoms including the SNF1 complexes in yeasts ...
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