Split each flask (from Step 3 ... feeder-containing cultures to ensure the quality of the feeder cells. We routinely culture HESCs in p60 dishes. This protocol is based on culturing HESCs in ...

While in vivo cells are protected by the immune system, there is no immune response in a tissue culture flask, so the cells are vulnerable to opportunistic pathogens and contaminants. Contamination ...

1 The 100-cm2 growth area of each OptiCell (33% more than a T-75 flask ... Cell Culture System (RC2S) capable of processing 402 OptiCells from inoculation to harvest with almost no human intervention.

According to Magnus, OTR is a proxy for how fast cells or bacteria breathe ... which uses a circulation pump to move the air in a shake flask past carbon dioxide and oxygen sensors.