The main steps in a western blot protocol are as follows. Sample preparation, including protein extraction and quantification Protein separation via gel electrophoresis Membrane transfer of proteins ...

A western blot is a laboratory method used to detect specific protein molecules from among a mixture of proteins. This mixture can include all of the proteins associated with a particular tissue ...

The BlotCycler automates western blot development and processing. BlotCycler, automated western processor, will process the blot through all washing, blocking, and incubation steps. Once the ...

She was using western blotting to study large ribosomal proteins, employing a protocol with two overnight steps. “Finally, I had that blot, and went to image it, but there was nothing there.

The multiplex fluorescent detection and chemiluminescent imaging capabilities of the FluorChem Q provide a complete solution for quantitative Western blot imaging and analysis. The open platform ...

It is important to understand the advantages and disadvantages of different ways of detecting a protein of interest on a western blot membrane. Here, we consider the three main types of protein ...