To carry out real-time PCR, researchers have to choose not only what primers to design (see Box 2) but also what detection chemistry to use. In many cases these decisions will be influenced ...

Extension: Nucleotides are added to the primers, which serve as extension points to continue new strand synthesis. Endpoint analysis: Scientists analyze PCR products typically using agarose gel ...

With the technique called polymerase chain reaction (PCR), scientists can make multiple copies of a specific genetic sequence within DNA. PCR is a powerful tool for researchers because it allows ...

Today’s thermocyclers are more intuitive and flexible, letting scientists focus on their experiments rather than their ...

also known as PCR clean-up, is the process of removing undesirable compounds and leftover reaction components, such as unincorporated deoxyribonucleotides (dNTPs), primers, primer dimers ...

PCR amplifies DNA in a three-step process: denaturation, which melts double-stranded DNA into single strands; annealing, which lets small pieces of primer DNA bind to either side of the region of ...

Since the emergence of COVID-19 in late 2019, the gold standard in testing for the disease has been a nuclear-derived technique: real-time reverse transcription–polymerase chain reaction, or real-time ...