The activated glutamine is coupled to the 5'-OH of the AD02 ribozyme; so, to achieve aminoacylation of tRNA in the second step, the authors had to perform several rounds of thermocycling to allow ...

Loss of TRMT10A led to a decrease in the levels of initiator methionine tRNA (tRNA iMet) and a specific glutamine tRNA (tRNA Gln). This reduction weakened the initiation of protein synthesis for ...