Based on CD16 and CD56 expression, the authors 1 then isolate three different ‘NK’ cell subsets and check CD107a expression in the three subpopulations. Here again, CD3+ cells are not gated ...

Degranulation was measured by the production of lysosomal-associated membrane protein-1 (CD107a) on NK cells in the presence of their target cells and was comparable in CD38 KO and CD38 WT cells.

NK cell degranulation was assessed via CD107a expression within the CD56+ cell population. *p<0.05. Image Credit: MaxCyte Inc. adapted from Carlsten et al. 2016 under Creative Commons License ...