Mammalian genomes are organized by multi-level folding, yet how this organization contributes to cell type-specific transcription remain unclear. We uncovered that the nuclear protein SATB1 ...

Mouse tissue samples (∼50 mg) underwent homogenization in 200 uL of ice-cold RIPA buffer (consisting of 150 mM NaCl, 1.0% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, and 50 mM Tris, pH 8.0) ...

Cells were lysed in RIPA buffer containing Tris (pH 7.4, 50 mM), NaCl (150 mM), 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS, and protease inhibitor. The concentrations of the extracted proteins were ...

Tissues or cells were homogenized and lysed with a buffer containing 0.1% SDS, 0.5% sodium deoxycholate, 1% NP-40, and a protease inhibitor mixture (PMSF, aprotinin, and sodium orthovanadate). Total ...

R-708-5p is a stress-regulated microRNA and a potential biomarker for the differential diagnosis of human mood disorders.