For all request of viral vectors, please visit the Gene Vector Core Website or send an email to genevector ... An SOP for each procedure has been published on this site. Cloning Vectors: Cloning is an ...

The VDL utilizes a vector construct that employs a nonhomologous viral envelope (derived from the vesicular ... The production of a lentivirus begins with cloning the gene of interest (goi) into an ...

This PCR kit, however, can be used for individual miR-30 shRNA cloning experiments with reasonable ... but is not essential. Prepare the vector DNA by digesting 2 μg of pSM2 with EcoRI and ...