This procedure includes fragmenting the DNA (sonication, nebulization or shearing), followed by DNA repair and end polishing (blunt end or A overhang) and, finally, platform-specific adaptor ligation.
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Sewing machines cost anywhere from around $100 to several thousand. Here's what you need to know to find a good one.
First wash off the foot, hand or other punctured skin with soap and water. Then soak the puncture wound in warm soapy water for 15 minutes. For any dirt or debris, gently scrub the wound surface back ...
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